Kottayam: The Rubber Research Institute of India at the Kottayam based Rubber Board under the Ministry of Commerce & Industry, Government of India, has launched a high-quality Sanger DNA sequencing service. The service may be availed by researchers, students and private firms.
Sequencing service will be provided for column-based purified PCR products and plasmid DNA samples using ABI 3500XL 24 Capillary Electrophoresis System. High Quality – DNA sequencing read lengths up to -1000 bases will be provided.
DNA sequence provides the order of the four nucleotides (building blocks) of the DNA molecule. The order, or sequence, of these blocks, tells your cells how to behave. Determination of DNA sequence is a basic and preliminary requirement of any molecular biology research. Ability to determine the DNA sequence laid the foundation of modern-day biology and revolutionized the biotechnology sector (Clinical, industrial and basic science). Sanger sequencing is one of the best methods for determining the nucleotide sequence of DNA and has been the powerhouse of DNA sequencing since its invention in 1970. It is considered the gold standard in sequencing due to its high accuracy of 99.99%. The method has been extensively used to advance the field of functional and comparative genomics, evolutionary genetics and complex disease research. Because of its suitability for routine validation of single genes or short DNA fragments, Sanger sequencing remains a popular technique and is widely used by researchers and industries working in the field of biotechnology. (Eg; sequencing of the spike protein from SARS-CoV-2).
DNA sequence reports will be sent by email. Orders may be placed online by filling the sample submission form and sending it to thomasku@rubberboard.org.in.
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